Influence of Electrical and Electromagnetic Stimulation on Nerve Regeneration in the Transected Mouse Sciatic Nerve : An Electron Microscopic Study

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Influence of electrical and electromagnetic stimulation on nerve regeneration was electron microscopically examined in the transected mouse sciatic nerve. Two days after the transection, several thin regenerating axons (daughter axons) were observed between the myelin sheath and basal lamina of Schwann cells in the proximal stump. Growth cones of the daughter axons contained several small round vesicles and mitochondria, and the shaft of them, neurofilaments, neurotubules and profiles of smooth-surfaced endoplasmic reticulum. The electrical and electromagnetic stimulation was applied to the proximal stump 2 days after the transection. At 3 and 6 hours after the electrical stimulation, some growth cones exhibited degenerative changes, such as swelling of mitochondria, rupture of small vesicles and formation of multivesicular bodies, but at 24 hours after the stimulation, these degenerative changes were hardly encountered. At 3 and 6 hours after the electromagnetic stimulation, most of the growth cones exhibited several multivesicular bodies, and at 24 hours after the stimulation, these degenerating growth cones were still encountered. These degenerative changes were not found in any axonal shaft of the daughter axons or parent axons encircled by myelin sheath. These findings suggest that the growth cones are very sensitive to the electrical and electromagnetic stimulation.
神戸大学の論文
2001-11-30