Interleukin-6 Facilitates Escherichia coli Transcytosis in the Caco-2 Cell Monolayer System

概要

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Aim : Bacterial translocation is a major concern as a cause developing sepsis or multiple organ failure under systemic inflammatory response with hypercytokinemia aroused from surgical stress. However, direct effect of interleukin 6 (IL-6) on bacterial translocation has rarely been reported. In this study, the effect of IL-6 on enterocyte with enterononinvasive E. coli interaction was evaluated in the intestinal epithelial cells. Methods : Direct effect of IL-6 on matured villus enterocytes, confluent Caco-2 cells on porous filter with double chamber system (Transwell), was evaluated by measuring permeability from apical (intestinal lumen side) to basolateral chamber (blood vessel side). Viable E. coli c-25 permeability, fluorescein sulfonic acid permeability, and transepithelial electrical resistance, LDH secretion, and ultrastructural analysis were performed under 100 ng/ml of IL-6 in the basolateral chamber medium. Results: IL-6 directly stimulated E. coli passage across Caco-2 monolayer cells by transcellular route with intact nuclear and cytoplasmic structure. Paracellular permeability change and cellular injury were not observed. It is suggested that IL-6 stimulates enterocytes from basolateral side, affects enterocyte-E. coli interaction, and increases E. coli transcytosis. Conclusions : IL-6 directly facilitates E. coli transcytosis in monolayer intestinal cells.
神戸大学の論文
1999-12-26