Analysis of the Essential Oil from Radix Bupleuri Using Capillary Gas Chromatography

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A simple and rapid capillary gas chromatographic (CGC) method with flame ionization detection has been newly developed for analysis of the essential oil from Radix Bupleuri. Twenty components were identified with gas chromatography-mass spectrometry. E-2-heptenal, furan, 2-pentyl, and E-2-nonenal were quantified simultaneously using the internal standard method. Decane was used as an internal standard. Separation and quantification were achieved on a DB-5 capillary column (30m×0.25mm i.d., 0.25-μm film thickness). The oven temperature was programmed as follows : 60℃ to 70℃ at 1℃/min rate, 70℃ for 10min, 3℃/min to 120℃, 20℃/min to 250℃, and held at 250℃ for 5min. The oven pressure was programmed as follows : 46.1kPa for 25min, 20.0kPa/min to 77.6kPa, and then held for 22min. Split injection was conducted with a split ratio of 10:1; flow-rate, 1.00ml/min; carrier gas, nitrogen; injector temperature, 280℃; and detector temperature, 280℃. The system proved effective in resolving E-2-heptenal, furan, 2-pentyl, and E-2-nonenal peaks from their interfering components. The method displayed excellent linearity in the range of 26.8-1072μg/ml (E-2-heptenal), 6.5-1292μg/ml (furan, 2-pentyl), and 7.8-1564μg/ml (E-2-nonenal). The average recovery rates of E-2-heptenal, furan, 2-pentyl, and E-2-nonenal were 100.3%, 102.8%, and 97%, respectively. CGC is a quick and accurate method for analysis of the essential oil from Radix Bupleuri.
公益社団法人日本薬学会の論文
2005-10-01

Analysis of the Essential Oil from Radix Bupleuri Using Capillary Gas Chromatography

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