HPLC Determination and Pharmacokinetic Study of Homoeriodictyol-7-O-β-D-glucopyranoside in Rat Plasma and Tissues(Analytical Biochemistry)

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Homoeriodictyol-7-O-β-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, ^^1H- and ^^<13>C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol-water-glacial acetic acid (45:55:0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1-200.0μg・ml^<-1> in rat plasma and 0.05-5.0μg・ml^<-1> in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from -0.8 to 5.4% in plasma and from -5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CL_<tot> were 16.04±3.19μg・h・ml^<-1> and 0.85±0.17 l・kg^<-1>・h^<-1>, respectively. T_<1/2,α> and t_<1/2,β> were 0.06±0.01h and 1.27±0.31h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine.
公益社団法人日本薬学会の論文
2007-04-01