バルビタールカルシウム緩衝液による牛血清蛋白質のセルロースアセテート膜電気泳動について

概要

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In order to improve the separativity of serum proteins of cattle in electro-phoresis on cellulose acetate membrane, the constituents and the concentrations of buffersolution were investigated in this experiment.Better results of fractionation were obtained from a barbital calcium buffer (pH 8.6)wltich consisted of 2.0 g of calcium lactate and l .0 Z of 0.06 M barbital buffer than from thestandard barbital buffer. By using that buffer, a-globulin (gl) in the serum of cattle wasdivided into two fractions, a.-gl and a.-gl, and the separation of $-gl and 7-gl was clearlyvisible.The percentages of the serum protein fractions in healthy dairy cattle were determinedand the normal range of each fraction was calculated. A significant increase in ex.- anda.-gl was recognized in various diseases, including liver disturbances, nephritis and someinfectious diseases.It is considered that in making the clinical diagnosis of some disease, the barbital calciumbuffer is worthy of applying to the fractionation of serum proteins of cattle by electro-phosesis with cellulose acetate membrane. By using this buffer, the serum protein fractionswere separated distinctly, and a.- and a.-gl divided clearly and increased significantly inseveral diseases.
社団法人日本獣医学会の論文
1974-08-25