グラジエントSDS-PAGEによる口蓋腺唾液のタンパクおよび糖タンパクバンドの分析

概要

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For the purpose of clarifying the relation between the retention and stability of the maxillary denture and proteins in palatine saliva, we have fractionated proteins in palatine saliva by means of 3〜l0% gradient SDS-PAGE method, and stained fractionated proteins including glycoproteins with silver. Thirty four samples of palatine saliva showed 27 protein bands, and fifteen samples of palatine saliva showed 12 glycoprotein bands. Estimated molecular weights of palatine saliva proteins were in a range from 33,000 to over 1,000,000, while those of glycoproteins were in a range from 40,000 to over 1,000,000. The glycoproteins which were thought to be most closely related to the retention and stability of the denture were clearly fractionated into 3 bands, and we named them bands 1a, 1b, 1d in order of their molecular weights. In the protein bands which showed high percentaze concentration were band 1a, 1b, 1d, 16b, 10. In the glycoprotein bands which showed high percentage were band 1a, 1b, 1d, 9, 16b. These six bands were thought to be major proteins conteined in palatine saliva.
日本補綴歯科学会の論文
1987-12-01