Effect of Abscisic Acid on Cell-Wall Metabolism in Guard Cells of Vicia faba L.
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概要
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Cell-wall synthesis in guard cells of Vicia faba L. was examined using sonicated epidermal strips incubated with [^<14>C]glucose. The cell walls of the guard cells incorporated [^<14>C]glucose at a lower level in the dark than in the light. Stomatal aperture in the epidermal strips was reduced by application of 1 μM abscisic acid (ABA) in the light but not in the dark. The ABA treatment reduced the incorporation of [^<14>C]glucose into the cell walls especially in the light. Fractionation of the labeled cell-wall components revealed that ABA inhibited the synthesis of pectic substances and cellulose, but did not affect hemicellulose synthesis. Microautoradiographs of the cell-wall fraction of the epidermal strips showed that a large amount of radioactivity was distributed at both ends of the guard cells in the absence of ABA and that removal of pectic substances from the cell-wall fraction resulted in uniform distribution of the radioactivity in the cell walls of the guard cells. These results indicated that the synthesis of pectic substances was active at both ends of the guard cells and was inhibited by ABA. Measurement of specific activities of neutral sugars in the guard-cell walls showed that polymers composed of galactose underwent active turnover and that synthesis of glucans was inhibited by ABA. These results revealed a strong correlation between the stomatal movement and the synthesis of pectic substances and cellulose in the guard cells, suggesting that the cell-wall metabolism in the guard cells may play a role in the regulation of stomatal movement.
- 日本植物生理学会の論文
著者
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Takeuchi Yuichi
Division Of Environmental Biology The National Institute For Environmental Studies
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Kondo Noriaki
Division Of Biosciences Graduate School Of Sciences And Engineering Teikyo University Of Science And
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