Plasma Membrane H^+-ATPase in Guard-Cell Protoplasts from Vicia faba L
スポンサーリンク
概要
- 論文の詳細を見る
The activity of plasma membrane H^+-ATPase was measured with membrane fragments of guard-cell protoplasts isolated from Vicia faba L. AT hydrolytic activity was slightly inhibited by oligomycin and ammonium molybdate, and markedly inhibited by NO^-_3 and vanadate. In the presence of oligomycin, ammonium molybdate and NO^-_3, the ATP-hydrolyzing activity was strongly inhibited by vanadate. It was also inhibited by diethylstilbestrol (DES), p-chloromercuribenzoic acid (PCMB) and Ca^<2+>, but slightly stimulated by carbonyl cyanide m-chlorophenylhydrazone (CCCP). The acitivity had higher specificity for AT as a substrate than other phosphoric esters such as ADP, AMP, GTP and p-nitrophenylphosphate; the K_m was 0.5 m_M for AT. The activity required Mg^<2+> but was not affected by K^+, and it was maximal around pH 6.8. When guard-cell protoplasts were used instead of membrane fragments, the ATPase activity reached up to 800 μmol Pi・(mg Chl)^<-1>・h^<-1> in the presence of lysolecithin. These results indicate that the guard cell has a high plasma membrane H^+_ATPase activity.
- 日本植物生理学会の論文
著者
-
Kondo Noriaki
Division Of Biosciences Graduate School Of Sciences And Engineering Teikyo University Of Science And
-
Shimazaki Ken-ichiro
Division Of Environmental Biology National Institute For Environmental Studies
関連論文
- Light-dependent Release of Carbon Metabolism Enzymes from Chloroplast Thylakoids Has No Relation to Light-activation of the Enzymes(Biological Chemistry)
- Polar and Neutral Lipid Changes in Spinach Leaves with Ozone Fumigation : Triacylglycerol Synthesis from Polar Lipids
- SO_2 Tolerance of Tobacco Plants Regenerated from Paraquat-Tolerant Callus
- NO_2 Suppression of Light-Induced Nitrate Reductase in SquaSh Cotyledons
- Change in Nitrate Reducing Activity in Squash Seedlings with NO_2 Fumigation
- Inhibition of Zeatin-Induced Growth of Cucumber Cotyledons by Sulfite Ions
- Photosynthesis-Dependent Volume Regulation in Guard Cell Protoplasts from Vicia faba L.
- High Respiratory Activity of Guard Cell Protoplasts from Vicia faba L.
- Effect of CO_2 on Volume Change of Guard Cell Protoplast from Vicia faba L.
- Photosynthetic Properties of Guard Cell Protoplasts from Vicia faba L.
- O_3 Tolerance and the Ascorbate-Dependent H_2O_2 Decomposing System in Chloroplasts
- Further Evidence for Inactivation of Fructose-1,6-bisphosphatase at the Beginning of SO_2 Fumigation : Increase in Fructose-1,6-bisphosphate and Decrease in Fructose-6-phosphate in SO_2-Fumigated Spinach Leaves
- Accumulation of Hydrogen Peroxide in Chloroplasts of SO_2-Fumigated Spinach Leaves
- Reversible Inhibition of the Photosynthetic Water-Splitting Enzyme System by SO_2-Fumigation Assayed by Chlorophyll Fluorescence and EPR Signal in Vivo
- Sulfite Inhibition of Photosystem II in Illuminated Spinach Leaves
- Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO_2-fumigated leaves of spinach
- Inhibition of Photosynthesis by Sulfite in Mesophyll Protoplasts Isolated from Vicia faba L. in Relation to Intracellular Sulfite Accumulation
- Outward-Rectifying K^+ Channels in Stomatal Guard Cell Protoplasts
- Intra-Vacuolar Reserves of Membranes During Stomatal Closure : The Possible Role of Guard Cell Vacuoles Estimated by 3-D Reconstruction
- Effect of Abscisic Acid on Cell-Wall Metabolism in Guard Cells of Vicia faba L.
- Effect of Abscisic Acid on Glucose Metabolism in Guard Cells of Vicia faba L.
- Effects of sulfite and pH on abscisic acid-dependent transpiration and on stomatal opening
- Inhibition site of the electron transport system in lettuce chloroplasts by fumigation of leaves with SO_2
- Plasma Membrane H^+-ATPase in Guard-Cell Protoplasts from Vicia faba L
- Abscisic Acid-Induced Stomatal Closure in Vicia faba Epidermal Strips. : Excretion of Solutes from Guard Cells and Increase in Elastic Modulus of Guard Cell Wall
- Specific inhibition of photosystem II activity in chlproplasts by fumigation of spinach leaves with SO_2
- Participation of Hydrogen Peroxide in the Inactivation of Calvin-Cycle SH Enzymes in SO_2-Fumigated Spinach Leaves