Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO_2-fumigated leaves of spinach
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概要
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Chlorophyll a and carotenoids of spinach began to be destroyed 2 to 3 hr after fumigation with 2 ppm SO_2 under light, whereas chlorophyll b was undamaged during 8 hr of exposure to SO_2. Pheophytin a was not affected by the fumigation. When disks excised from leaves fumigated with SO_2 at 2 ppm for 2 hr were illuminated, chlorophyll a and carotenoids were broken down, while they were not destroyed in darkness. The destruction of these pigments was suppressed under nitrogen. Chlorophyll a destruction was inhibited by 1,2-dihydroxybenzene-3,5-disulfonate (tiron) , hydroquinone and ascorbate, but not by 1,4-diazabicyclo-[2,2,2]-octane (DABCO), methio-nine, histidine, benzoate and formate. Chlorophyll a destruction was inhibited by phenazine methosulfate but stimulated by methyl viologen. Addition of superoxide dismutase (SOD) to the homogenate of SO_2-fumigated leaves inhibited the chlorophyll a destruction. The activity of endogenous SOD was reduced to 40% by 2-hr fumigation before the loss of chlorophyll was observed. These results suggest that chlorophyll a destruction by SO_2 was due to superoxide radicals (O_2^-). Moreover, malondialdehyde (MDA), a product of lipid peroxidation, was formed in SO_2-fumigated leaves. MDA formation was inhibited by tiron, hydroquinone and DABCO but not by benzoate and formate. MDA formation was increased by D_2O. These results suggest that lipid peroxidation in SO_2-fumigated leaves was due to singlet oxygen (^1O_2) produced from O_2^-.
- 日本植物生理学会の論文
著者
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Kondo Noriaki
Division Of Environmental Biology The National Institute For Environmental Studies
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Kondo Noriaki
Division Of Biosciences Graduate School Of Sciences And Engineering Teikyo University Of Science And
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Sugahara Kiyoshi
Division of Environmental Biology, The National Institute for Environmental Studies
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Shimazaki Ken-ichiro
Division of Environmental Biology, The National Institute for Environmental Studies
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Sakaiki Takeshi
Division of Environmental Biology, National Institute for Environmental Studies
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Sugahara Kiyoshi
Division Of Environmental Biology The National Institute For Environmental Studies
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Sugahara Kiyoshi
Division Of Enviromental Biology The National Institute For Enviromental Studies
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Shimazaki Ken-ichiro
Division Of Environmental Biology The National Institute For Environmental Studies
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Shimazaki Ken-ichiro
Division Of Environmental Biology National Institute For Environmental Studies
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Sakaiki Takeshi
Division Of Environmental Biology National Institute For Environmental Studies
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