Participation of Hydrogen Peroxide in the Inactivation of Calvin-Cycle SH Enzymes in SO_2-Fumigated Spinach Leaves
スポンサーリンク
概要
- 論文の詳細を見る
In SO_2-fumigated spinach leaves under light, chloroplast SH enzymes, glyceraldehyde-3-phosphate dehydrogenase (NADP-GAPD) (EC 1.2.1.13), ribulose-5-phosphate kinase (Ru5PK) (EC 2.7.1.19) and fructose-1,6-bisphosphatase (FBPase) (EC 3.1.3.11) were more remarkably inactivated than other chloroplast enzymes. Their activities recovered after removal of SO_2. The inactivation paralleled light-dependent CO_2-fixation in spinach leaves. In illuminated chloroplast isolated from SO_2-fumigated spinach leaves, NADP-GAPD and Ru5PK were more specifically inactivated than other chloroplast enzymes. These two enzymes could be protected from the inactivation by adding catalase. The NADP-GAPD inactivation was suppressed by DCMU, cytochrome c or anaerobic conditions. BY adding thiol compounds, the NADP-GAPD inactivation was discharged and the activity increased. In chloroplasts or crude extracts from non-fumigated spinach leaves, NADP-GAPD and Ru5PK were more strongly inhibited by externally added H_2O_2 than other chloroplast enzymes. All results supported the idea that the suppression of photosynthesis at the beginning of results supported the idea that the suppression of photosynthesis at the beginning of SO_2 fumigation was caused by the reversible inhibition of chloroplast SH enzyme with H_2O_2.
- 日本植物生理学会の論文
著者
-
Kondo Noriaki
Division Of Biosciences Graduate School Of Sciences And Engineering Teikyo University Of Science And
-
Tanaka Kiyoshi
Division Of Environmental Biology National Institute For Environmental Studies
-
Otsubo Tetsuaki
Department Of Environmental Chemistry Faculty Of Science Toho University
関連論文
- Ozone Tolerance and Glutathione Reductase in Tobacco Cultivars(Biological Chemistry)
- Light-dependent Release of Carbon Metabolism Enzymes from Chloroplast Thylakoids Has No Relation to Light-activation of the Enzymes(Biological Chemistry)
- Water Deficiency-induced Changes in the Contents of Defensive Substances against Active Oxygen in Spinach Leaves(Biological Chemistry)
- Differences between Spinach and Kidney Bean Plants in Terms of Sensitivity to Fumigation with NO_2
- Polar and Neutral Lipid Changes in Spinach Leaves with Ozone Fumigation : Triacylglycerol Synthesis from Polar Lipids
- SO_2 Tolerance of Tobacco Plants Regenerated from Paraquat-Tolerant Callus
- NO_2 Suppression of Light-Induced Nitrate Reductase in SquaSh Cotyledons
- Change in Nitrate Reducing Activity in Squash Seedlings with NO_2 Fumigation
- Inhibition of Zeatin-Induced Growth of Cucumber Cotyledons by Sulfite Ions
- Photosynthesis-Dependent Volume Regulation in Guard Cell Protoplasts from Vicia faba L.
- High Respiratory Activity of Guard Cell Protoplasts from Vicia faba L.
- Effect of CO_2 on Volume Change of Guard Cell Protoplast from Vicia faba L.
- Photosynthetic Properties of Guard Cell Protoplasts from Vicia faba L.
- O_3 Tolerance and the Ascorbate-Dependent H_2O_2 Decomposing System in Chloroplasts
- Further Evidence for Inactivation of Fructose-1,6-bisphosphatase at the Beginning of SO_2 Fumigation : Increase in Fructose-1,6-bisphosphate and Decrease in Fructose-6-phosphate in SO_2-Fumigated Spinach Leaves
- Accumulation of Hydrogen Peroxide in Chloroplasts of SO_2-Fumigated Spinach Leaves
- Reversible Inhibition of the Photosynthetic Water-Splitting Enzyme System by SO_2-Fumigation Assayed by Chlorophyll Fluorescence and EPR Signal in Vivo
- Sulfite Inhibition of Photosystem II in Illuminated Spinach Leaves
- Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO_2-fumigated leaves of spinach
- Electronic Structure of a Cluster Taken from High-T_c Superconductor of YBa_2Cu_3O_7: (Cu_4O_2)(O_4Ba)(Cu_4O_4)Y(Cu_4O_4)(O_4Ba)(Cu_4O_2) Embedded in Ionic Cage of Point Charges
- Inhibition of Photosynthesis by Sulfite in Mesophyll Protoplasts Isolated from Vicia faba L. in Relation to Intracellular Sulfite Accumulation
- Intra-Vacuolar Reserves of Membranes During Stomatal Closure : The Possible Role of Guard Cell Vacuoles Estimated by 3-D Reconstruction
- Effect of Abscisic Acid on Cell-Wall Metabolism in Guard Cells of Vicia faba L.
- Effect of Abscisic Acid on Glucose Metabolism in Guard Cells of Vicia faba L.
- Role of superoxide dismutase in defense against SO_2 toxicity and an increase in superoxide dismutase activity with SO_2 fumigation
- Effects of sulfite and pH on abscisic acid-dependent transpiration and on stomatal opening
- Plasma Membrane H^+-ATPase in Guard-Cell Protoplasts from Vicia faba L
- Abscisic Acid-Induced Stomatal Closure in Vicia faba Epidermal Strips. : Excretion of Solutes from Guard Cells and Increase in Elastic Modulus of Guard Cell Wall
- Participation of Hydrogen Peroxide in the Inactivation of Calvin-Cycle SH Enzymes in SO_2-Fumigated Spinach Leaves
- Photosynthetic Activity in the Developing Cotyledon of Soybean Seeds