Glutamine synthesis in gelminating castor bean endosperm
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概要
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The pathway of glutamine synthesis in germinating castor bean endosperm was investigated by feeding experiments with (2,3-^<14>C)succinate and by determining enzyme activities related to pyruvate formation and utilization. ^<14>C of (2,3-^<14>C)succinate was rapidly and sequentially incorporated into amino acids in the following order : aspartate or alanine, glutamate and glutamine. ^<14>CO_2 was slowly released, especially during the early hours of incubation. Fluorocitrate inhibited ^<14>CO_2 release while aminooxyacetate stimulated it slightly. Fluorocitrate inhibited the incorporation of ^<14>C into glutamate and glutamine. Aminooxyacetate inhibited ^<14>C incorporation into aspartate, alanine, glutamate and glutamine. Glutamine synthetase activity was detected in a soluble fraction. NAD-malic enzyme activity was detected in mitochondria by sucrose density gradient centrifugation. Activities of pyruvate decarboxylase and aldehyde dehydrogenase were detected. Aldehyde dehydrogenase was partially purified about 60-fold by ammonium sulfate fractionation and the DEAE-cellulose chromatography. The Km values of the enzyme were 0.71 m_M for NAD and 0.43 m_M for acetaldehyde. Based on these results and properties of pyruvate kinase reported previously (9), the metabolism of pyruvate in cytosol and mitochondria was discussed in connection with glutamine synthesis in germinating castor bean endosperm.
- 日本植物生理学会の論文
著者
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Nakayama Hitoshi
Department of Pharmacology, Nara Medical University School of Medicine, Japan
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Miura Kazuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Nakayama Hitoshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Fujii Michiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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