The light-induced oxidation-reduction reaction of cytochrome b-559 in membrane fragments of the blue-green alga Anabaena variabilis
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概要
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Light-induced oxidation-reduction reactions of cytochrome b-559 were investigated with membrane fragments of Anabaena variabilis and supplementarily with Plectonema boryanum. The oxidation-reduction reactions of cytochrome b-559 observed with membrane fragments were similar in their kinetics to those of the cytochrome in aged chloroplasts. The reactions were annihilated by the addition of Ferro, indicating that the cytochrome of high redox potential (E'_0 = + 373 mV pH 6.5) was photoreduced and oxidized. Titration with reducing agents indicated that cytochrome b-559 is contained in Anabaena membrane fragments in an amount 1.5 times as much as the content of P700 on a molar basis; the content of the species of high redox potential was estimated to be around 70%. Kinetic treatment of the photoreduction indicated that the cytochrome was reduced at some site of the electron transport system between the two photosystems. The photo-oxidation depended on the action of either photosystem II or I even in the presence of DCMU, indicating that the photooxidation was induced by both photosystems. The oxidation by photosystem I action was inhibited by HgCl_2-treatment, indicating that this reaction is mediated by plastocyanin. EDTA (5 × 10^<-3> M) suppressed the cytochrome photoreduction and enhanced the rapid phase of the photooxidation. The latter effect appeared only when an appropriate dark time (3 min) was inserted after the cytochrome photoreduction. The phenomenon was interpreted as showing that EDTA modifies the reactivity of the electron carrier which directly donates electrons to cytochrome b-559. The oxidation, and probably also the reduction of cytochrome b-559, was assumed to be regulated by the oxidation-reduction state of this carrier.
- 日本植物生理学会の論文
著者
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Fujita Yoshihiko
Ocean Research Institute University Of Tokyo
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Fujita Yoshihiko
Ocean Research Instrtute Umverslty Of Tokyo
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