Photochemical activity fluorescence and absorption spectrum of the photochemically active chromoprotein (ACP) isolated from the blue-green alga Anabaena cylindrica
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概要
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Photochemical, spectroscopic and fluorescence characteristics of the active chromoprotein (ACP) of Anabaena cylindrica were studied with preparations having different spectroscopic characteristics. Purified ACP preparations occasionally showed spectroscopic characteristics differing considerably from those of ordinary ACP. A spectroscopic difference in these preparations was observed only in the relative absorbance in the 620 nm peak. Absorption peaks at 430, 460, 480 and 695 nm remained unchanged. Photochemical activity and fluorescence yield at 700 nm were fairly constant in these preparations, when excited at absorption bands other than 620 nm. Spectroscopic changes caused by heat and detergent (SDS) treatments occurred only in the relative absorbance of the 620 nm peak. Photochemical and fluorescence characteristics of the treated ACP were the same as those of the ACP originally having a different absorption spectrum. A comparison of various ACP preparations indicates that ACP is a complex of two pigments, c-phycocyanin and a pigment with a 695 nm absorption maximum. The reduction in the molecular size of ACP by SDS-treatment indicates that pigment 695 is far smaller in its molecular size than c-phycocyanin. A possible function of the two pigments in the photochemical reaction is also discussed.
- 日本植物生理学会の論文
著者
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Fujita Yoshihiko
Ocean Research Institute, University of Tokyo
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Fujita Yoshihiko
Ocean Research Institute University Of Tokyo
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Suzuki Reiko
Ocean Research Institute University Of Tokyo
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Pjon Che-jun
Ocean Research Institute University Of Tokyo
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