The separation and characterization of the photoreactive component of a photochemically active chromoprotein (ACP) obtained from the blue-green alga Anabaena cylindrica
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概要
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A photoreactive component, P690, was isolated from ACP by successive treatments with SDS and Triton X 100. The isolated P690 is a chromoprotein with a molecular weight of 50,000 daltons and an isoelectric point at pH 9.1. Its spectrum has absorption maxima at 275, 450, 475, 630 and 690 nm and it emits, at - 196℃, a fluorescence at 695 nm. The photo-chemical oxidation-reduction reaction activity is fully retained in the P690. The isolated P690 forms a complex with phycocyanin in vitro. This complex was identical with the original ACP in all characteristics examined. The complex formation of P690 is specific to phycocyanin. A possible mechanism for ACP formation is discussed.
- 日本植物生理学会の論文
著者
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Fujita Yoshihiko
Ocean Research Institute University Of Tokyo
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Pjon Che-jun
Ocean Research Institute University Of Tokyo
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