Studies on the Hill reaction of membrane fragments of blue-green algae I. Stabilizing effect of various media on the 2,6-dichlorophenol indophenol-Hill activity of membrane fragments obtained from Anabaena cylindrica and Anabaena variabilis
スポンサーリンク
概要
- 論文の詳細を見る
The stability of DPIP-Hill activity of membrane fragments from blue-green algae was investigated in various suspension media. Two blue-green algae, Anabaena cylindrica and A. variabilis were used. Activities for the DPIP-Hill reaction were stable in the two algal preparations only when preparations were suspended in a medium containing high concentrations of carbohydrates or PEG. Required concentrations of carbohydrates or PEG differed greatly in the two preparations; 1.5 M sucrose or 20% PEG for A. cylindrica and 0.3 M sucrose or 6% PEG for A. variabilis were minimal for maintaining full activity in 2 hr incubation at O℃, respectively. Below these concentrations, activities decreased rapidly. In both preparations, stabilizing effects, on a molar basis, varied in different kinds solutes. However, a simple relation was found between the effects and water concentrations of media. In A. cylindrica, a 50% decay in activity occurred in 2 hr incubation at O℃ in a medium with 43.8 M water, and, in A. variabilis, the water concentration was 53.4 M. In media free of carbohydrate or PEG, Mg^<++> ion had a moderate stabilizing ability. EDTA acted antagonistically to Mg^<++>. Effective concentrations were the same in two preparations. However, in the medium containing carbohydrate or PEG, their actions were insignificant. Results suggest that the molecular organization in membranes necessary for Hill reaction is easily destroyed under high water concentrations, and that added solutes stabilize the activity by reducing water concentrations.
- 日本植物生理学会の論文
著者
-
Fujita Yoshihiko
Ocean Research Institute University Of Tokyo
-
Suzuki Reiko
Ocean Research Institute University Of Tokyo
関連論文
- Comparison of main emissions at -196℃ from phycobilisomes of two blue-green algae Anabaena cylindrrca and Anacystis nidulans
- Quantitative relationship between two reaction centers in the photosynthetic system of blue-green algae
- Studies on Morphological Changes of the Blue-green Alga Nostoc muscorum A with Special Reference to the Role of Light
- Action of Near UV and Blue Light on the Photocontrol of Phycobiliprotein Formation : A Complementary Chromatic Adaptation
- Photoreversible absorption changes of guanidine-HCl-treated phycocyanin and allophycocyanin isolated from the blue-green alga Tolypothrix tenuis
- In vivo transformation of phycobiliproteins during photobleaching of Tolypothrix tenuis to forms active in photoreversible absorption changes
- Photocontrol of phycoerythrin formation in the blue-green alga Tolypothrix tenuis growing in the dark
- On the Relationship between Photocontrol of Phycoerythrin Formation and Photoreversible Pigment of Scheibe
- Studies on the Hill reaction of membrane fragments of blue-green algae V. A correlation between the solubilization of a photochemically active chromoprotein (ACP) and the inactivation of photosystem II activity in membrane fragments of Anabaena cylindrica
- Photochemical activity fluorescence and absorption spectrum of the photochemically active chromoprotein (ACP) isolated from the blue-green alga Anabaena cylindrica
- The separation and characterization of the photoreactive component of a photochemically active chromoprotein (ACP) obtained from the blue-green alga Anabaena cylindrica
- Pyrone derivatives : effective inhibitors of photosynthetic electron flow system
- Light-dependent oxygen uptake by non-living, chlorophyll-containing particles obtained from oceanic environments
- A probable lack of function of c-type cytochrome in the photosynthetic system of the blue-green alga Anabaena variabilis
- Changes in photosynthetic activity of the diatom Phaeodactylum tricornutum in a culture of limited volume
- Studies on the Hill reaction of membrane fragments of blue-green algae I. Stabilizing effect of various media on the 2,6-dichlorophenol indophenol-Hill activity of membrane fragments obtained from Anabaena cylindrica and Anabaena variabilis
- Immunological identification of pigment component of a photochemically active chromoprotein (ACP) isolated from the blue-green alga Anabaena cylindrica
- Ascorbate photo-oxidation by a photochemically active chromoprotein isolated from the blue-green alga Anabaena cylindrica : the effect of monochromatic illumination
- Carotenoid photobleaching induced by the action of photosynthetic reaction center II : DCMU-sensitivity
- Studies on the Hill reaction of membrane fragments of blue-green algae II. The reaction steps inactivated at high water concentration
- Studies on the Hill reaction of membrane fragments of blue-green algae IV. Carotenoid photobleaching induced by photosystem II action
- The light-induced oxidation-reduction reaction of cytochrome b-559 in membrane fragments of the blue-green alga Anabaena variabilis
- Electron donor-specificity observed in photosystem I reactions of membrane fragments of the blue-green alga Anabaena variabilis and the higher plant Spinacea oleracea
- Studies on the Hill reaction of membrane fragments of blue-green algae III. Fluorescence characteristics of membrane fragments of Anabaena variabilis and Anabaena cylindrica
- Phycoerythrin of the marine blue-green alga Trichodesmium thiebautii
- The C550 photoresponse at room temperature observed in membrane fragments of the blue-green alga Anabaena variabilis
- Carotenoid photobleaching induced by photosystem II action in the membrane fragments of the blue-green alga Anabaena variabilis : Action of O_2