Changes in Histone H1 Phosphorylation during Differentiation of Mouse Myeloid Leukemia Cells

概要

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The possibility that phosphorylation of protein (s) is related to the differentiation of mouse myeloid leukemia cells (M1 cells) was examined by comparing phosphoproteins in M1 cells and in M1 cells treated with dexamethasone for various lengths of time. For this purpose, M1 cells or M1 cells treated with dexamethasone were incubated with [^<32>P] H_3PO_4 in vivo and fractionated into subcellular fractions, then phosphoproteins were analyzed by gel electrophoresis followed by autoradiography. The analyses revealed no changes either in proteins detectable with Coomassie blue staining or in phosphoproteins except H1 histone at the early stage of differentiation of M1 cells. Phosphorylation of H1 histone in M1 cells occurred almost exclusively on H1B. The amount of H1B decreased remarkably after dexamethasone treatment, whereas the H1B phosphorylation level increased significantly between 16 and 48h after dexamethasone treatment and thereafter decreased.
公益社団法人日本薬学会の論文
1984-06-25