Fluorometric High Performance Liquid Chromatographic Analysis of Prostaglandin (PG) Metabolites : Application to the Catabolism of PGE_2 and PGF_<2α> in Rat and Rabbit Kidney Homogenates
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概要
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A previously reported fluorometric high performance liquid chromatographic procedure for separation of 9-anthryldiazomethane (ADAM) derivatives of prostaglandins (PG's) was applied to examine catabolic products of PG's, such as 15-keto-PGE_2,13,14-dihydro-15-keto-PGE_2,15-keto-PGF_<2α>, and 13,14-dihydro-15-keto-PGF_<2α>. ADAM derivatives of these metabolites were separated in 30 min on an ODS column with 65% acetonitrile. By this method catabolism of PGE_2 and PGF_<2α> in kidney homogenates of rats and rabbits was analyzed. Homogenates of medulla and cortex of rat kidney catabolized PGE_2 and PGF_<2α> to 15-keto-PG's. Whereas the homogenate of rabbit kidney cortex yielded 13,14-dihydro-15-keto-PGE_2,that of medulla did not. The conversion of PGF_<2α> to PGE_2 was shown to occur in homogenates of rabbit kidney cortex as well as rat kidney medulla and cortex.
- 公益社団法人日本薬学会の論文
著者
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Ohishi Sachiko
Department Of Pharmacology School Of Pharmaceutical Sciences Kitasato University
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Ohishi Sachiko
Department Of Pharmacology Kitasato University School Of Pharmaceutical Sciences
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Yamaki Kohji
Department Of Pharmacology School Of Pharmaceutical Sciences Kitasato University
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YAMAKI KOHJI
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University
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