Effect of Alloxan on the Incorporation of Uridine Diphospho-D-Galactose into Cultured Pancreatic Endocrine Cells of the Rat

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The effect of alloxan on cultured rat pancreatic endocrine cells was investigated by following changes in the incorporation of UDP-galactose into glycoproteins (chloroform/methanol-insoluble fraction) and glycolipids (chloroform/methanol-soluble fraction). Cultured cells exposed to either alloxan or alloxan plus D-glucose anomers (16.7 mM) at 37° for 5 min, were incubated in a medium containing labeled UDP-galactose for 60 min. Alloxan at concentrations of 1.25 or 6.25 mM had no marked effect on the incorporation of UDP-galactose but 31.2 mM alloxan markedly inhibited the incorporation. The combination of 1.25 mM alloxan and the α anomer of D-glucose significantly reduced the incorporation of UDP-galactose into glycoproteins and glycolipids, while the presence of the β anomer with alloxan had no effect. Incorporation of D-[U-^<14>C] galactose was unaffected by 1.25 mM alloxan or alloxan plus D-glucose anomers (16.7 mM). The present data indicate that cultured rat pancreatic endocrine cells can transfer galactose from UDP-galactose and that the α anomer reinforces the action of alloxan to a greater extent than the β anomer.
社団法人日本薬学会の論文
1980-07-25