Insulin Release from the Perifused Isolated Rat Langerhans Islets under a Slow-rise Glucose Stimulation

概要

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The dynamics of insulin release from the perifused rat Langerhans islets was investigated under a slow-rise and square-wave stimulation by glucose. Every dose response curve were sigmoidal profile, in which the threshold of glucose was shown to be around 4.2 mM. The K_m values of dose response curves were similar each other under a slow-rise and square-wave stimulation by glucose. Since a rise of gradient level from 0.10 to 0.22 mM/min did not alter the dose response curve, it was proved that a profile of insulin release in response to slowly rising glucose was not due to a lag time in insulin release but dependent to an absolute change of glucose level. The stimulation by rapid change of glucose level with 2.80 mM/min of gradient level caused an insulin releasing profile similar to that by the square-wave stimulation. Consequently, it was suggested that the insulin release under a slow-rise stimulation by glucose at gradient levels around 0.10-0.22 mM/min was more physiologic. This stimulation method is an easily available procedure for preparing accurate dose response relationship between the rate of insulin released and the concentration of glucose.
公益社団法人日本薬学会の論文
1977-08-25