高速液体クロマトグラフ法による医薬品の分析(第6報)リュウタンおよびゲンチアナ中のゲンチオピクロサイドの迅速定量

概要

論文の詳細を見る
A method for the rapid estimation of gentiopicroside in crude drugs, Gentianae scabrae radix and Gentianae radix, was established by the use of high-speed liquid chromatography. Gentiopicroside is separated on the 25 cm column of Zorbax ODS, using a liquid chromatograph (Shimadzu-Du Pont Model LC-3A) with 5% tetrahydrofuran as the desorption solution, and the separation is completed within 8 min. Gentiopicroside in the crude drugs is extracted with water and the aqueous extract is injected into the column. The content of gentiopicroside was calculated from calibration curves previously prepared using the standard. Precision of the determination is about ±1% and detection limit is 2 ng (S/N ratio, 3). This method is considered to be useful for the evaluation of these crude drugs.
公益社団法人日本薬学会の論文
1979-10-25