高速液体クロマトグラフィーによるアルブチン及びヒドロキノンの分別定量法の人尿及びラット血しょうへの応用の基礎的研究

概要

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A rapid and sensitive method using high-speed liquid chromatography was devised for the determination of arbutin and hydroquinone in human urine and rat plasma. The procedure involves Amberlite XAD-2 column chromatographic separation from human urine sample and methanol extraction from rat plasma sample, respectively. The water effluent or methanol extract is injected into the chromatograph, with a Zorbax CN column(4.6 mm i. d. ×25cm), water as mobile phase, a flow rate of 1.0ml/min, and a column temperature of room temperature. Arbutin and hydroquinone are detected by their absorbance at 221nm and quantitated by measuring peak heights. Separation of both compounds is achieved within 10min. The limits of detection were 2 μg/ml of human urine and 1μg/ml of rat plasma, respectively. The recovery of both compounds from urine and plasma was (98〜101) %, and the coefncient of variation (0.80〜0.92) %, respectively.
社団法人日本分析化学会の論文
1979-12-05