高速液体クロマトグラフィーによるペオニフロリン及び安息香酸の分別定量法の血しょう及び血清への応用の基礎的検討

概要

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A rapid and sensitive method using high-performance liquid chromatography was devised for the determination of paeoniflorin and benzoic acid in rat plasma and bovine serum (reference control serum). Separation was achieved within 12 min on a Zorbax CN column, using 0.1 M HClO_4(pH 3.5)-CH_3CN(9:1, v/v) as an eluant with a Shimadzu LC-2 liquid chromatograph. Recovery in this method was about 100 %. Paeoniflorin and benzoic acid in plasma and serum were extracted with methanol and the extract was applied to the Zorbax CN column. The limit of detection of paeoniflorin and benzoic acid was about 3 μg and 0.6 μg in 1 ml plasma and serum, respectively (S/N ratio, about 3).
社団法人日本分析化学会の論文
1980-10-05