Whole Body Autobacteriographyによるブドウ球菌のマウス生体内分布に関する研究

概要

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Whole body autobacteriography was proposed as an experimental method to follow distribution and localization of pathogenic microbes in experimentally infected animals. In the present studies, experimental staphylococcosis in mice was studied by this method. Mice were infected with Smith -diffuse strain of Staphylococcus aureus by intravenous or intraperitoneal route. Whole body sections of 40 μ in thickness were obtained in sterile condition with a cold microtome at various intervals after infection. These sections were immediately (or after freeze drying) placed on agar plates and incubated at 37°over-night. The sections were then treated with triphenyltetrazolium chloride for vital staining. The autobacteriograms after intravenous infection demonstrated a rapid disappearance of the microbes from the blood stream and their accumulation in the spleen, liver and lung. They also revealed the extensive staphylococcal multiplication in the kidney, causing abscess 24 hours later. These informations do not conflict with the results obtained by usual microbiological techniques. The distribution and localization of staphylococci in mice infected intraperitoneally were also evaluated by autobacteriography when mice were treated with tetracycline or penicillin immediately after infection. The advantages of this method are discussed.
公益社団法人日本薬学会の論文
1970-09-25