生薬の規格設定に関する研究(第18報) : セネガサポニンの理化学的定量法

概要

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The quantitative assay of Senega saponin was performed by measuring ultraviolet (UV) absorption. Substances A and B, isolated from Senega saponin by thin-layer chromatography (TLC) and silicagel column chromatography, showed the following physical properties : A : mp 252-253° (decomp.) UV λ^<MeOH>_<max> : 315 mμ B : mp 256-257° (decomp.) UV λ^<MeOH>_<max> : 315 mμ Maximum absorption at 315 mμ may be due to the presence of cinnamic acid in saponin molecule. Thus the quantitative determination of Senega saponin was carried out by isolation of A and B on TLC (with their recognition by UV lamps, followed by measuring their UV absorption coefficients after followed by recognition of the two spots with a UV lamp, extraction of their areas and measurement of their UV absorption coefficients). Values of saponin in Senega root from various sources obtained by this method were in the range of 8-16%. These values were comparable with those obtained by the hemolytic index method. (This method was also correlated with the hemolytic index method.)
公益社団法人日本薬学会の論文
1969-08-25