Aspergillus Proteinaseに関する研究(第3報) : Aspergillus melleus Proteinaseの物理化学的性質ならびにアミノ酸組成について酵素剤の研究第39報

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Some physicochemcial properties and amino acid compositions of the Aspergillus melleus proteinases were investigated. 1) Semi-alkaline proteinase from Aspergillus melleus : The molecular weight was 30300 using the Svedverg's formula, based upon the values of the sedimentation coefficient (S^0_<20,w>=2.98S), the diffusion coefficient (D^0_<20,w>=8.75×10^<-7> cm^2/sec), and the partial specific volume (V=0.728). The enzyme contains 16.35% of nitrogen and is composed of 314 residues of amino acid ; Asp. 36,Glu. 18,Ser. 37,Thr. 25,Gly. 45,Ala. 46,Val. 33,Leu. 21,Ileu. 15,Pro. 9,Phe. 7,Tyr. 11,Trp. 2,Arg. 2,Lys. 5,His. 2,(amide-NH_3)16. 2) Alkaline proteinase from Aspergillus melleus : The molecular weight was 27900 using the Svedverg's formula, based upon the value of the sedimentation coefficient (S^0_<20,w>=2.85S), the diffusion coefficient (D^0_<20,w>=9.05×10^<-7> cm^2/sec), and the partial specific volume (V=0.726). The enzyme contains 16.10% of nitrogen and is composed of 282 residues of amino acid ; Asp. 33,Glu. 16,Ser. 33,Tyr. 17,Gly. 45,Ala. 39,Val. 22,Leu. 16,Ileu. 10,Pro. 6,Phe. 5,Tyr. 15,Trp. 3,Arg. 5,Lys. 12,His. 5,(amide-NH_3)16.
公益社団法人日本薬学会の論文
1968-12-25