カエル卵レクチンの抗腫瘍性(第2報)ニホンアカガエル卵レクチンの静脈注射後局所血行一時停止法による抗腫瘍作用の増強

概要

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Effect of egg lectin from Rana japonica (RJ-lectin) on the growth of Ehrlich solid tumor was examined in male ddY mice, using the temporary circulation-stop method as an in vitro model. Lectin was injected intravenously 24 hr after inoculation of Ehrlich ascites carcinoma (1×10^6 cells) into the right thigh. The upper thigh was tightly ligated to stop blood circulation, and the animals were kept without movement with warming of the leg at 37° for 30 min. The antitumor activity was estimated by weihing the solid tumor on the 10th day after cell inoculation. Intravenous administration of RJ-lectin in the temporary circulation-stop method more strongly suppressed the tumor growth than by intravenous injection of the lectin without any treatment. The inhibitory action of RJ-lectin in the temporary circulation-stop method depended on the number of administrations. The inhibition rate of the lectin at 10 or 20 mg/kg (three intravenous administrations) was 93 and 77%, respectively. In this method the lectin may easily contact with tumor cell as observed in vitro and is able to react with the cells directly for a long time. It is suggested that RJ-lectin suppresses the tumor growth by direct cytotoxic effect on tumor cells.
公益社団法人日本薬学会の論文
1978-07-25