無麻酔無傷のラットを用いた新しい胃酸分泌測定方法

概要

論文の詳細を見る
Gastric acid secretion was repeatedly measured in the same rats which were conscious and unoperated. A vescical catheter for female use (No. 4) was introduced into the stomach of fasted rats via the oesophagus. Two milliliters of saline were injected through this tube and about 1.5ml was rapidly aspirated. Acid in the aspirate was titrated with 1/100 N NaOH. This procedure were repeatedly at 15,30 or 60 min intervals. It was possible that gastric acid secretion was measured repeatedly 4 times in 2 weeks in the same rats. Antisecretory activities of atropine and cimetidine, and secretagogue actions of tetragastrin and histamine, but not bethanechol and carbachol, were detected by this method. This method should be available for the study of the pharmacological evaluation of antiulcerative drugs as well as the gastric acid secretory mechanisms.
1984-10-25