急性肝外性胆管閉塞ラット肝におけるリソゾームの変化

概要

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In order to clarify the lysosomal change of the rat liver in acute extrahepatic biliary obstruction, the activities of N-acetyl-β-glucosaminidase (NAG) and acid phosphatese (AcP) in the liver and serum were measured over a period until 7 d after the bile duct ligation (BDL). Liver lysosomal change on the biochemical basis was examined by using the liver 3 d after BDL treatment. From the results of the differential centrifugation experiments, the activities of the above enzymes in the nuclear fraction increased significantly, while those in the lysosomal fraction decreased. Sucrose density gradient centrifugation distribution patterns demonstrate that the lysosomal enzyme activities in the nuclear fraction tended to decrease, while those in the lysosomal fraction increased significantly. From kinetic parameters after BDL treatment, K_m value in the nuclear fractions was in agreement with that in the lysosomal fractions. On the other hand, a significant increase in V_<max> values in the nuclear fraction was found. From these results, it is considered that lysosomal particles are apt to be aggregated and that the size is larger in the liver after the BDL treatment.
公益社団法人日本薬学会の論文
1984-01-25