酸素分子の活性化及び活性酸素に対する生体防御機構に関する研究
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概要
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One of the active oxygen species, superoxide (O_2^-), was generated by the electrolytic reduction of molecular oxygen in acetonitrile. O_2^- was determined by the ultraviolet (UV) (λ_<max>/nm=255,ε=1.48×10^3 M^<-1>cm^<-3>) and the electron spin resonance (ESR) (g⫽=2.083,g_⊥=2.008) spectrum. O_2^- could easily react with tocopherols (vitamin E and its derivatives) to give the corresponding chromanoxyl radicals of which structures were determined by ESR. ESR studies of the reactions of O_2^- with tocopherols or their model compounds indicate that the radical concentrations from tocopherol models correlate with the physiological activities of the tocopherols. O_2^- could also react with some biologically active quinones such as vitamin K_8 and vitamin E quinone to give the corresponding semiquinone radicals. The fact that vitamin E quinone, an irreversible metabolite of vitamin E, was reduced by O_2^- to the semiquinone radical suggests that, like vitamin E, vitamin E quinone may also scavenge O_2^- and protect living cells from the effects of O_2^- in a hydrophobic environment. Further, O_2^- could react with some metalloporphyrins. In this case, non-redox metalloporphyrins such as Zn (II) TPP (TPP : tetraphenylporphine), Cd (II) TPP, Mg (II) TPP generated the superoxide adduct by the reaction with O_2^-. On the other hand, redoxactive metalloporphyrins such as Cr (III) TPP・Cl, Mn (III) TPP・Cl, Co (II) TTP (TTP : tetra-p-tolylporphine) and Co (III) TTP・Cl underwent the addition and/or redox reactions with O_2^-. Another active oxygen species, hydroxyl radical (OH・), was first detected from some copper (II) coplexes such as Cu (en)_2 (en : ethylenediamine) with hydrogen peroxide (H_2O_2) by ESR spin trapping and thiobarbituric acid (TBA) methods. Further, by using Cu (en)_2-H_2O_2 system the most active OH・scavenger was determined. This Cu (en)_2-H_2O_2 system will be useful for determing the antioxidant ability against OH・.
- 公益社団法人日本薬学会の論文
- 1990-09-25
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