ネズミチフス菌TA1535/pSK1002を用いた大気浮遊粉じん試料の変異原性試験法の検討

概要

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It was investigated whether or not the umu test, a new screening method for mutagens by use of Salmonella typhimurium TA1535/pSK1002,was applicable to organic extracts from airborne particulates. First, when nutritious medium was generally used in the test, the mutagenic activities were found to be rather weak in the case of both the extracts with S9 mix and 2-nitrofluorene without S9 mix. However, these values turned out to increase by a revised method by using a simple medium restricted nutritionally ; this method had a similar sensitivity to Ames test for the extracts and 2-nitrofluorene ; there arose adequate dose-response relationships between amounts of the extracts and their mutagenic activities in the presence and absence of S9 mix. It was therefore suggested that this revised method could be applied to the extracts to assay these mutagenicity due to its improved sensitivity in addition to easiness and quickness.
公益社団法人日本薬学会の論文
1985-04-30