ガスクロマトグラフィー/マススペクトロメトリーによる2型及び3型プロスタノイドの分離条件の検討とエイコサペンタエン酸(EPA)摂取により生成する3型プロスタノイドへの応用

概要

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Gas chromatographic (GC) separating conditions for 2- and 3- series of prostanoids were investigated by using prostaglandin F_<2α> (PGF_<2α>) and PGF_<3α> as model compounds. The combination of the dimethylisopropylsily (DMIPS) ether derivatives and the capillary column coated with a polar liquid stationary phase of 50%-phenylmethylsilicone enabled to separate them with the difference of about 0.3 methylene unit values. This condition was applied for GC/mass spectrometric analysis of the metabolites of eicosapentaenoic acid (EPA), demonstrating the obvious separation of Δ^<17>-6-keto-PGF_<1α> and 11-dehydrothromboxane B_3 from the corresponding 2-series metabolites originated from arachidonic acid.
公益社団法人日本薬学会の論文
1991-04-30