Calcium Signals in Helper T Cells after Interaction with Antigen-Specific and Antigen-Nonspecific B Cells

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概要

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• By confocal fluorescence microscopy we have studied the rises of the intracellular free calcium ion concentration ([Ca^<2+>]_i) in helper T cells (KLH-specific, I-A^k-restricted Th1 cells, 28-4) after interaction with antigen-specific and antigen-nonspecific B cells (antigen-presenting cells). Antigen-specific and antigen-nonspecific B cells were prepared by the preincubation of TNP (trinitrophenol)-specific B cell hybridomas (TP67.21 I-A^k) with TNP-conjugated KLH and KLH alone, respectively. Calcium signals in Th1 cells (28-4) were induced by antigen-specific B cells one hundred times more efficiently as those by antigen-nonspecific B cells. Herbimycin A, a tyrosine kinase inhibitor, suppressed the former signals but not the latter. These results indicated that tyrosine phosphorylation was involved in the antigen processing of antigen-specific B cells but not in the processing of antigen-nonspecific B cells.

• 公益社団法人日本薬学会の論文
• 1996-05-15

著者

• NAKANISHI Mamoru

  Faculty of Pharmaceutical Sciences, Nagoya City University

• Asano Y

  Osaka Univ. Osaka Jpn

• NAGAO Yoko

  Faculty of Pharmaceutical Sciences, Nagoya City University

• NOJI Masahide

  Faculty of Pharmaceutical Sciences, Nagoya City University

• ASANO Yoshihiro

  School of Medicine, University of Tokyo

• HAMANO Teruaki

  Hyogo College of Medicine

• Nagao Yoko

  Faculty Of Pharmaceutical Sciences Nagoya City University

• Hamano T

  Hyogo College Of Medicine

• Asano Yoshihiro

  School Of Medicine University Of Tokyo

• Noji Masahide

  Faculty Of Pharmaceutical Sciences Nagoya City University

• Nakanishi Mamoru

  Faculty Of Pharmaceutical Science University Of Tokyo

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