Monosialoganglioside Containing Cationic Liposomes with a Cationic Cholesterol Derivative Promote the Efficiency of Gene Transfection in Mammalian Culture Cells

概要

論文の詳細を見る
We have studied the effects of monosialoganglioside (GM_1)-containing cationic liposomes with a cationic cholesterol on the liposome-mediated gene transfection into mammalian culture cells. The results showed that both cationic liposomes with either a cationic cholesterol derivative of a hydrophobic amino head group (I) and a hydrophilic amino head group (II) promoted the transfection of luciferase plasmids (pGL3) into HeLa and CHO-K1 cells more than the control cationic liposomes without GM_1. In addition, we found that cationic liposomes with a cationic cholesterol derivative (II) were about ten times as effective as that by commercially available cationic liposome Lipofectin. Confocal fluorescence microscopy showed that the liposome/DNA comples was transferred more efficiently into the target cells by the GM_1-containing liposomes thant by the liposomes without GM_1. In proportion to the above results, free antisense DNAs were also more efficiently transferred into the nucleus of the target cells by the GM_1-containing liposomes. When there was 100 mM galactose in the transfection medium, the luciferase activity by the GM_1-containing liposomes was reduced to the level of the control liposomes. The results suggest that GM_1-containing cationic liposomes with a cationic cholesterol derivative of a hydrophobic amino head group or a hydrophilic amino head group should significantly increase the transfection efficiency of plasmid DNAs and antisense DNAs by galactose receptor-mediated endocytosis. This means that the GM_1-containing liposomes described here should be very promising for gene transfection in vitro.
公益社団法人日本薬学会の論文
2000-06-01

Monosialoganglioside Containing Cationic Liposomes with a Cationic Cholesterol Derivative Promote the Efficiency of Gene Transfection in Mammalian Culture Cells

スポンサーリンク

概要

著者

関連論文

スポンサーリンク