A Simple Quantitative Measurement of mRNA of Human β-actin by Reverse Transcription Competitive PCR with a Compact Digital Camera
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概要
- 論文の詳細を見る
Reverse transcription (RT) competitive polymerase chain reaction (PCR) is a sensitive and useful technique for the absolute quantitation of mRNA level. We developed a competitive PCR method with a compact digital camera DC 40 (Eastman Kodak Company, NY, U.S.A.) for the measurement of human β-actin cDNA (mRNA), a reference gene in an analysis for target gene expression. The devised method, compared with currently used methods, was easy, safe, sensitive, and highly reproducible to assay an infinitesimal level of cDNA (mRNA).
- 公益社団法人日本薬学会の論文
- 1997-09-15
著者
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Nagai Hiroshi
Department of Cardiology, Regeneration Medicine and Bioethics, Gifu University Graduate School of Me
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OKAMOTO Yoshihiro
Department of Health Promotion and Preventive Medicine, Nagoya City University Graduate School of Me
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Nishida Mikio
Department Of Clinical Pharmacy Faculty Of Pharmacy Meijo University
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Nagai Hiroshi
Department Of Cardiology Regeneratiion Medicine And Bioethics Gifu University Graduate School Of Med
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Nishida M
Meijo Univ. Nagoya Jpn
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HARA Yusuke
Department of Clinical Pharmacy, Faculty of Pharmacy, Meijo University
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KATOH Yayoi
Department of Clinical Pharmacy, Faculty of Pharmacy, Meijo University
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Katoh Yayoi
Department Of Clinical Pharmacy Faculty Of Pharmacy Meijo University
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Nakamoto H
Chugai Pharmaceutical Co. Ltd. Tokyo Jpn
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Okamoto Yoshihiro
Department Of Clinical Pharmacy Faculty Of Pharmacy Meijo University
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Hara Y
Denso Corp. Aichi Jpn
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Hara Yusuke
Department Of Anesthesia Kyoto University Hospital
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Nishida Mikio
Department Of Biochemistry Faculty Of Pharmaceutical Sciences University Of Tokushima
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Nagai Hiroshi
Department Of Anatomy Okayama University Sohool Of Dentistry
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Nagai Hiroshi
Department Of Anatomy Okayama University School Of Dentistry
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Okamoto Yoshihiro
Department of Chemistry, Faculty of Engineering Science, Osaka University
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