Determination of the Functional Domain of a Mouse Autonomous Replicating Sequence
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概要
- 論文の詳細を見る
We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7,1-6,1987). Here we report the nucleotide sequence of ARS65. The sequence from BglII to EcoRI sites cloned as ARS was 2568 bp long. There exist three intersting domains : a TA repeat, a myc like box (essential sequence for c-myc ARS, and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hydromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
- 公益社団法人日本薬学会の論文
- 1997-06-15
著者
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Ogata Masanori
Department Of Physiology School Of Allied Health Sciences Kitasato University
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Ogata Masanori
Research Center Shinagawa Refractories Co. Ltd.
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Ogata Masanori
Research Institute Daiichi Seiyaku Co. Ltd.
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ARIGA Hiroyoshi
Faculty of Pharmaceutical Sciences, Hokkaido University
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IGUCHI-ARIGA Sanae
College of Medical Technology, Hokkaido University
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Fujino Hiromichi
Laboratory Of Chemical Pharmacology Graduate School Of Pharmaceutical Sciences Chiba University
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Fujino Hiromichi
Faculty Of Pharmaceutical Sciences Hokkaido University
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Iguchi-ariga Sanae
College Of Medical Technology Hokkaido University
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Ariga Hiroyoshi
Faculty Of Pharmaceutical Sciences Hokkaido University
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HAYASHI Chiharu
Faculty of Pharmaceutical Sciences, Hokkaido University
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SATO Yoshinori
Research Institute, Daiichi Seiyaku Co., Ltd.,
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Hayashi C
Faculty Of Pharmaceutical Sciences Hokkaido University
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Sato Yoshinori
Research And Development Center Hayashibara Biochemical Laboratories Inc.
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