Cloning and Sequencing of the astA Gene Encoding Arylsulfate Sulfotransferase from Salmonella typhimurium
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概要
- 論文の詳細を見る
Arylsulfate sulfotransferase (ASST) transfers a sulfate group from a phenolic sulfate ester to a phenolic acceptor substrate. In the present study, the gene encoding ASST was cloned from a genomic library of Salmonella typhimurium. The gene was subcloned into the vector pKF3 and was sequenced. A recombinant clone harboring the gene was directly identified using a fluorescent assay. Sequencing revealed two contiguous open reading frames (ORFs) on the same strand. Based on amino acid sequence homology, ORF1 and ORF2 are designated as astA and dsbA, respectively. The deduced amino acid sequence of astA from S.typhimurium was highly similar to those of the Enterobacter amnigenus, Klebsiella, and Campylobacter jejuni ASSTs, encoded by the astA genes. However, an ASST activity assay revealed a different acceptor specificity. Using p-nitrophenyl sulfate (PNS) as a donor substrate, phenol is the best acceptor substrate, followed by α-naphthol, resorcinol, tyramine, acetaminophen, and tyrosine.
- 公益社団法人日本薬学会の論文
- 2001-05-01
著者
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Kwon A‐r
Seoul National Univ. Seoul Kor
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Kim D‐h
Kyung Hee Univ. Seoul Kor
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Kim D‐h
College Of Pharmacy Kyung-hee University
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KANG Jin-Wook
College of Pharmacy, Seoul National University
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KWON Ae-Ran
College of Pharmacy, Seoul National University
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KIM Dong-Hyun
Korea and College of Pharmacy, Kyunghee University
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CHOI Eung-Chil
College of Pharmacy, Seoul National University
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Choi E‐c
Seoul National Univ. Seoul Kor
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Choi Eung-chil
College Of Pharmacy Seoul National University
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Kang Jin-wook
College Of Pharmacy Seoul National University
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Kim Dong-hyun
Korea And College Of Pharmacy Kyunghee University
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