Release of Intermediate Reactive Hydrogen Peroxide by Macrophage Cells Activated by Natural Products
スポンサーリンク
概要
- 論文の詳細を見る
By determining the hydrogen peroxide (H_2O_2) released in cultures of peritoneal macrophage cells from Swiss mice, we evaluated the action of 27 vegetable compounds (pristimerin, tingenone, jatrophone, palustric acid, lupeol, cladrastin, ocoteine, boldine, tomatine, yohimbine, resepine, escopoletin, esculine, plumericin, diosgenin, deoxyschizandrin, p-arbutin, mangiferin, and others) using a 2mg/ml solution of each compound (100μg/well). Macrophages are cells responsible for the development of the immunological response reaction, liberating more than one hundred compounds into the extracellular environment. Among these are the various cytokines and the intermediate compounds of nitrogen (NO) and oxygen (H_2O_2). This coordinated sequence of biochemical reactions is known as the "oxidative burst." When we compared the results with those obtained with zymosan (an important stimulator of H_2O_2) we observed that the compounds showing the highest activity were substances 2 (tingenone), 16 (reserpine) and 20. Other substances such as compounds 1, 4, 5, 6, 8, 12, 13, 14, 15, 17, 19, 23, 24, 26, and 27 also showed a certain activity, but with less intensity than the aforementioned ones. Compounds 3, 7, 9, 10, 11, 18, 21, 22 and 25 presented no activity. These results suggest that natural products (mainly tingenone and reserpine and others) with different chemical structures are strong immunological modulators. However, further tests are needed to determine the 'oxidative burst' in future studies.
- 公益社団法人日本薬学会の論文
- 2001-02-01
著者
-
Vilegas Wagner
Departamento De Quimica Orĝanica Instituto De Quimica Universidade Estadual Paulista (unesp)
-
Vilegas Wagner
Departamento De Quimica Organica Instituto De Quimica Universidada Estadual Paulista
-
MOREIRA Raquel
Departamento de Principios Ativos Naturais e Toxicologia Brasil, Faculdada de Ciencias Farmaceuticas
-
CARLOS Iracilda
Departamento de Analises Clinicas, Faculdada de Ciencias Farmaceuticas, Universidada Estadual Paulis
-
Moreira Raquel
Departamento De Principios Ativos Naturais E Toxicologia Brasil Faculdada De Ciencias Farmaceuticas
-
Carlos Iracilda
Departamento De Analises Clinicas Faculdada De Ciencias Farmaceuticas Universidada Estadual Paulista
関連論文
- Flavonoids and a Naphthopyranone from Eriocaulon ligulatum and Their Mutagenic Activity
- Evaluation of Strychnos pseudoquina ST. HIL. Leaves Extract on Gastrointestinal Activity in Mice
- Antiulcerogenic Activity of Four Extracts Obtained from the Bark Wood of Quassia amara L. (Simaroubaceae)
- Effects of Tea from Turnera ulmifolia L. on Mouse Gastric Mucosa Support the Turneraceae as a New Source of Antiulcerogenic Drugs
- Constituents and Antiulcer Effect of Alchornea glandulosa : Activation of Cell Proliferation in Gastric Mucosa during the Healing Process(Pharmacology)
- Gastric Antiulcer Activity of Syngonanthus arthrotrichus SILVEIRA (Pharmacology)
- Determination of herbicides and a metabolite in human urine by liquid chromatography-electrospray ionization mass spectrometry
- Release of Intermediate Reactive Hydrogen Peroxide by Macrophage Cells Activated by Natural Products
- Studies on Anti-Allergic Constituents in the Leaves and Stems of Anchietia salutaris var. martiana (Violaceae)
- Inhibition of Hydrogen Peroxide, Nitric Oxide and TNF-α Production in Peritoneal Macrophages by Ethyl Acetate Fraction from Alchornea glandulosa(Miscellaneous)
- A New Cytotoxic Naphthoquinone from Paepalanthus latipes
- Effect of the Isocoumarin Paepalantine on the Luminol and Lucigenin Amplified Chemiluminescence of Rat Neutrophils
- Inhibition of nitric oxide and tumour necrosis factor-α production in peritoneal macrophages by Aspergillus nidulans melanin