A Chinese Traditional Medicine, Juzentaihoto, Inhibits the O^-_2 Generation by Macrophages

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Guinea pig peritoneal macrophages generate superoxide anion (O^-_2), when stimulated with N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), phorbol-myristate acetate (PMA) or ovalbumin complex of guinea pig IgG2 antibody (OAγ_2). These responses were found to be inhibited by a Chinese traditional medicine, Juzentaihoto (JT). When the inhibition was assayed by the use of fMLP as a stimulant, at least one of the substances responsible for the inhibitory activity of JT was identified to be cinnamic acid. An authentic sample of cinnamic acid also inhibited the O^-_2 generation by fMLP-stimulated macrophages. Cinnamic acid, however, did not inhibit the O^-_2 generation, when macrophages were stimulated with PMA and OAγ_2. These results indicate that a certain cinnamic acid-inhibitable factor may be involved in the intracellular triggering event (s) initiated by fMLP, leading to activation of the respiratory burst reduced nicotinamide adenine dinucleotide phosphate oxidase, but not in those by PMA and OAγ_2.
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