An Enzyme-Linked Immunosorbent Assay for the Detection of Plasminogen Activator-Induced Fribrin Clot Lysis in a Circulating Plasma System
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概要
- 論文の詳細を見る
A technically easy and sensitive method for the evaluation of the fibrinolytic potency of plasminogen activator in a circulating plasma system was developed. A circulating apparatus was prepared by connecting silicone tubes with two separate chambers. Human plasma and plasminogen activator were allowed to circulate from one chamber through a peristaltic pump into another one which contained biotin-labeled fibrin clot. The extent of fibrinolysis was evaluated on streptavidin-coated microtiter plates by measuring the amount of the avidin-bound fibrin degradation products with peroxidase-linked antibody. The detection limit of the fibrinolytic products was 1.5ng/ml. The present method dose not require any specialized equipment and is also applicable to a low-cost routine measurement of the thrombus selectivity of plasminogen activator.
- 公益社団法人日本薬学会の論文
- 1992-02-25
著者
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坂口 真也
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Fukuoka University
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永松 淳雄
福岡大学薬学部
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添田 秦司
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University
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占野 廣司
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University
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永松 淳雄
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University
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添田 秦司
福岡大学 薬学部 生化学
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