Effects of Metal Elements on β-Hexosaminidase Release from Rat Basophilic Leukemia Cells(RBL-2H3)

概要

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Immediate allergy is caused by a chemical mediator released from basophile and mast cells via cell degranulation due to reaction betweeen an immunoglobulin E (IgE) antibody, bound with the IgE receptor on the cell membrane, and an antigen. The present authors have established a new method for assaying the enzyme activity of β-hexosaminidase as an index of chemical mediator release. Using cultured cells instead of conventional methods based on histamine release from mast cells, the present method permits highly accurate mass screening since it uses a well-established cell line of rat basophilic leukemia cells(RBL-2H3). The effects of metal elements on immediate allergic reaction were evaluated using a newly developed assay system.A total of 38 metal elements were investigated for effects on immediate allergic reactions in vitro. These elements were classified by five types on the basis of action on β-hexosanimidase release : 1) those which showed very strong inhibitory action, such as ZnCl_2 and ZrCl_4,2) those which showed relatively strong inhibitory action, such as CdCl_2 and CuCl_2,3) those which showed relatively weak inhibitory action, such as CoCl_2 and Pb(NO_3)_2,4) those which showed neither inhibitory nor promoting action, such as MnCl_2 and SrCl_2,and 5) AgNO_3,which alone showed promoting action.
公益社団法人日本薬学会の論文
1991-08-25