The Effect of Staurosporine on Ca^<2+> Signals in Rat Basophilic Leukemia (RBL-2H3) Cells

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We examined the effect of staurosporine on cytosolic calcium response in rat basophilic leukemia (RBL-2H3) cells using fura-2 as a fluorescent indicator of calcium ion.Staurosporine at a dose of 30 nM inhibited antigen-stimulated Ca^<2+>-influx into the cells from the extracellular environment. In contrast, the drug at this concentration inhibited neither the mobilization of Ca^<2+> from intracellular stores nor inositol 1,4,5-trisphosphate (IP_3) formation. At a high concentration (300 nM), however, staurosporine completely inhibited the cytosolic calcium responses as well as IP_3 formation. These results indicate that staurosporine, if used at an appropriate concentration, can be used to discriminate Ca^<2+>-influx from extracellular environment from mobilization of the ion from intracellular stores.These results also suggest that protein kinases, possibly protein kinase C, are involved in the calcium influx of RBL-2H3 cells from the extracellular environment.Serotonin release was strongly inhibited by the drug at 30 nM staurosporine. Since the inhibition of serotonin release and suppression of cytosolic calcium increase in response to the antigen were in parallel, we concluded that the inhibition of serotonin release from RBL-2H3 cells caused by the drug was elicited by the suppression of Ca^<2+> influx into the cells.
公益社団法人日本薬学会の論文
1991-03-25