Transcellular Transport of Low Density Lipoprotein through Cultured Newborn Rat Skin Epidermal Cell Monolayer

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Epidermal cells from newborn rat skin were cultured on type IV collagen-coated Millipore filter, and the transport of low density lipoprotein (LDL) labeled with Rhodamine B isothiocyanate (RB-LDL) through the cultured cell layer was examined. The transport of RB-LDL was dependent on temperature and biological energy. The transport was low at 17℃, but above 20℃, it became high with increase in temperature up to 37℃. The transport was markedly inhibited by the energy inhibitors 2-deoxygluose and NaN_3. Furthermore, the transport was saturable at the RB-LDL concentration of about 300 μg/ml and the activation energy of the transport was determined as 104.6 kJ/mol. No degradation product of LDL (apoprotein B) was observed during LDL transport through the cultured cell layer. The transport of RB-LDL through skin epidermal cells in culture is suggested to be mediated by transcytotic vesicles, but not by endocytosis and exocytosis via the lysosomal system, nor through cellular junctions.
1991-02-25