Purification and Characterization of a Nuclease from Lentinus edodes
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概要
- 論文の詳細を見る
An endonuclease with 3'-nucleotidase activity (nuclease Le1) was purified from fruit bodies of Lentinus edodes in a single band on sodium dodecylsulfate-polyacrylamide gel electrophorosis (SDS-PAGE). The apparent molecular weight of nuclease Le1 was about 27000. The nuclease was inactivated in the presence of ethylenediaminetetraacetic acid (EDTA) and reactivated by the addition of Zn^<2+>. Hydrolysis of poly U by the nuclease showed many intermediate size oligomers prior to the formation of 5'-uridine monophosphate (UMP). Therefore, it was concluded that nuclease Le1 was a Zn^<2+>-endonuclease similar to P1-nuclease from Penicillium citrinum. The nuclease was very sensitive to ionic strength, but pH-profiles of the hydrolysis of four 3'-nucleotides were very similar to those of P1 nuclease from P. citrinum.
- 社団法人日本薬学会の論文
- 1991-10-25
著者
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入江 昌親
Department of Microbiology, Hoshi College of Pharmacy
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井口 法男
Department of Microbiology, Hoshi College of Pharmacy
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小山 隆
Department of Pharmacy, College of Science and Technology, Nihon University
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島田 弘子
Department of Pharmacy, College of Science and Technology, Nihon University
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入江 昌親
星薬大・微生物
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入江 昌親
Department Of Chemistry Institute For Infectious Diseases University Of Tokyo
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島田 弘子
Department Of Microbiology College Of Pharamacy
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井口 法男
日大・薬・微生物
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小山 隆
日大・薬・微生物
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Koyama T
Shinshu Univ. Nagano Jpn
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