Interaction of Cytidylyl (3'→5') cytidylyl (3'→5') cytidylyl (3'→5') guanylic Acid with Ribonuclease T_1 diazotized with Diazonium 1-(H)-tetrazole

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概要

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• Ribonuclease T_1 modified with diazonium-1-(H)-tetrazole (DHT-RNase T_1) reported by Kasai has been known to be inactive towards RNA, but active towards guanylyl (3'→5')-cytidine (GpC). In order to explain this peculiar phenomenon, a couple of experiments were performed. DHT-RNase T_1 is almost inactive towards poly G and poly C as well as RNA, but active against GpCpC. Michaelis constants of DHT-RNase T_1 for GpC was about twice as large as that of native RNase T_1. Although Ki value for 2', (3')-GMP of DHT-RNase T_1 was about 4 times as large as that of native RNase T_1,that of (Cp)_3Gp for DHT-RNase T_1 was more than 60 times larger than that for the native enzyme. From the results described above, it was suggested that a peculiar nature of DHT-RNase T_1 towards RNA was probably due to the unfavorable effect of the modification on the binding of guanylic acid residue having polynucleotide at 5'-OH side.

• 公益社団法人日本薬学会の論文
• 1976-09-25

著者

• 入江 昌親

  Department of Microbiology, Hoshi College of Pharmacy

• 入江 昌親

  Department Of Chemistry Institute For Infectious Diseases University Of Tokyo

• 入江 昌親

  Department Of Microbiology Hoshi College Of Pharmacy

関連論文

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