Purification, and Properties of a Base Non-specific Acid Ribonuclease from Bullfrog (Rana catesbeiana)
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概要
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An acid ribonuclease (RNase RCL_2) was purified to nomogeneity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) from a homogenate of bullfrog liver (Rana catesbeiana). The apparent molecular weight estimated from SDS-PAGE was ca. 25kDa. The pH optimum of the RNase was 5.0. The RNase released mononucleotides from RNA in the order of 3'-UMP, 3'-GMP and 3'-AMP. The N-terminal amino acid sequence of RNase RCL_2 was determined up to the 20th residue, and it was found to have a 5 residue sequence homology with that of oyster acid RNase [H. Watanabe et al. J. Biochem. (Tokyo), 114,800 (1993)]. Thus, RNase RCL_2 seems to be a member of the RNase T_2 family RNase. This is the first evidence of the RNase T_2 family RNase in amphibians.
- 社団法人日本薬学会の論文
- 1995-02-15
著者
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入江 昌親
Department of Microbiology, Hoshi College of Pharmacy
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井口 法男
Department of Microbiology, Hoshi College of Pharmacy
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小山 隆
Department of Pharmacy, College of Science and Technology, Nihon University
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入江 昌親
星薬大・微生物
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入江 昌親
Department Of Chemistry Institute For Infectious Diseases University Of Tokyo
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井口 法男
日大・薬・微生物
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小山 隆
日大・薬・微生物
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小林 弘子
日大・薬・微生物
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八木 弘光
Department of Microbiology, College of Pharmacy, Nihon University
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小林 弘子
Department of Microbiology, College of Pharmacy, Nihon University
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八木 弘光
Department Of Microbiology College Of Pharmacy Nihon University
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