Partial Purification of Ribonuclease from Trichoderma koningii

スポンサーリンク

概要

• 論文の詳細を見る

• Three ribonucleases (RNases) were partially purified from Trichoderma koningii by means of ammonium sulfate fractionation, column chromatographies on Amberlite IRC-50,Sephadex G-100,phospho-cellulose or diethylaminoethyl (DEAE)-cellulose and Sephadex G-75. Three RNases fractions, fraction I-III, are non-specific in respect of bases. Fraction I and II released nucleotides from ribonucleic acid (RNA) in the order of A>U>G>C and fraction III released nucleotides in the order of G>A>U>C. Molecular weight of three fractions estimated by gel filtration was 24000,18000 and 6000,respectively. pH optimum of fractions I and II was at pH 4.3 and that of fraction III was at pH 4.8. Cu^<2+>, Zn^<2+> and Hg^<2+> inhibited fractions I and II at the concentration of 10^<-3>M, but fraction II moderately. Mg^<2+> had no effect on the enzymatic activity of fraction I and II, but it accelerated fraction III activity considerably.

• 社団法人日本薬学会の論文
• 1974-08-25

著者

• 入江 昌親

  Department of Microbiology, Hoshi College of Pharmacy

• 入江 昌親

  星薬科大

• 入江 昌親

  Hoshi College of Pharmacy

• 原田 正富

  The Faculty of Pharmaceutical Sciences, Kyoto University

• 原田 正富

  The Faculty Of Pharmaceutical Sciences Kyoto University

• 入江 昌親

  Department Of Microbiology Hoshi College Of Pharmacy

関連論文

• Amino Acids and Peptides. XXI. : Synthesis of N-Terminal Heptapeptide of Mammalian Metallothionein (MT) and Evaluation of Its Immunoreactivity
• ウシガエル卵由来レクチン/シアル酸複合体のX線結晶構造解析
• Amino Acids and Peptides. XII. Synthesis of C-Terminal Decapeptide of Bovine Pancreatic Ribonuclease A (RNase A) and Its Analogs and Determination of Their Ability to Reactivate Des (121-124) RNase A
• Different Binding Behavior to Chitin of Multiple Forms of Glucoamylases from Aspergillus saitoi and Rhizopus sp.
• Different Behavior towards Raw Starch of Two Glucoamylases from Aspergillus saitoi
• Differentiation of Two Types of Tryptophan Residues Related to Enzymatic Activity in a Glucoamylase from a Rhizopus sp. by N-Bromosuccinimide Oxidation in the Presence of Tris(hydroxymethyl)aminomethane and Maltitol
• Oxidation of Glucoamylase from a Rhizopus sp. with N-Bromosuccinimide
• Properties of Rhizopus sp. Glucoamylase Polymerized by Crosslinking with Glutaraldehyde
• Kinetic and Circular Dichroism Spectroscopic Comparison among Three Forms of Glucoamylase from a Rhizopus sp.
• Subsite Affinity of a Glucoamylase from Aspergillus saitoi
• Proteolytic Modification of a Glucoamylase from a Rhizopus sp.
• Interaction of Several Drugs with Tyr 411 Anthraniloyl Human Serum Albumin
• N-Bromosuccinimide-oxidized Human Serum Albumin as a Tool for the Determination of Drug Binding Sites of Human Serum Albumin
• アルカリプロテアーゼによる毛髪の特異的分解
• リボヌクレアーゼについて
• Partial Purification of Ribonuclease from Trichoderma koningii
• 235 Aspergillus saitoiのリボヌクレアーゼの活性中心についての若干の知見
• ウシリボヌクレア-ゼのサブサイト
• Amino Acids and Peptides. XXXIII. Synthesis of N-Terminal Epitope Peptides of Mammalian Metallothioneins (MTs)
• Purification, and Properties of a Base Non-specific Acid Ribonuclease from Bullfrog (Rana catesbeiana)
• Purification and Characterization of a Nuclease from Lentinus edodes
• Reinvestigation of N-Bromosuccinimide Oxidation of Glucoamylases from Aspergillus saitoi
• The Modification of Tyrosyl Residues of a Minor Ribonuclease from Aspergillus saitoi
• The Effect of Elimination of Amino Acids from the Carboxyl-terminal of the Minor Ribonuclease from Aspergillus saitoi by Carboxypeptidase A on the Enzymatic Activity
• Purification and Characterization of a Nucleaes (3'-Nucleotidase) from a Penicillum sp.
• Isolation and Characterization of Protease Modified Ribonucleases from Rhizopus sp.(Biological,Chemical)
• Amino Acids and Peptides. XIV. Synthesis and Biological Activity of Three S-Peptide Analogues of Bovine Pancreatic Ribonuclease A (RNase A)(Organic,Chemical)
• Some Properties of Ribonucleases from Aspergillus saitoi and Seminal Vesicles immobilized on Sepharose 4B activated by Cyanogen Bromide
• Studies on the Specificity of Ribonuclease from Rhizopus sp.
• ムラサキウニ(Anthocidaris crassispina)およびバフンウニ(Hemicentrotus pulcherrimus)のオスとメスのRNaseの性質と役割について(一般講演4,第27回麻布環境科学研究会講演要旨)
• Carboxymethylation of a Ribonuclease from Rhizopus sp.
• Evidence for the Presence of a Histidine Residue having pK_a 7 in the Active Site of a Ribonuclease from a Rhizopus sp.
• Interaction of Cytidylyl (3'→5') cytidylyl (3'→5') cytidylyl (3'→5') guanylic Acid with Ribonuclease T_1 diazotized with Diazonium 1-(H)-tetrazole
• グルコアミラ-ゼの多様化について
• タイトル無し

もっと見る 閉じる

スポンサーリンク