Specific Release fo Rat Liver MIcrosomal Catalase

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The recovery of catalase in the microsomal fraction of rat liver was influenced by the solution used for preparation. When microsomes were prepared with TMKS-solution (50 mM Tris-HCl, pH 7.4,5 mM MgCl_2,25 mM KCl and 0.25 M sucrose), the catalase content of the microsomes (TMKS-microsomes) was about 50% of that in the microsomes (PS-microsomes )prepared with PS-solution (10 mM phosphate buffer, pH 7.0,and 0.25 M sucrose).Upon washing of PS-microsomes with 0.25 M sucrose containing 50 mM Tris, about 30% of catalase as well as fatty acyl-CoA oxidase, both being peroxisomal enzymes, was released. On the other hand, membrane marker enzymes, glucose-6-phosphatase and nucleoside diphosphatase, and a secretory albumin, were not substantially released by this treatment. No effect was observed on the release of catalase by washing PS-microsomes with 0.25 M sucrose containing MgCl_2 or KCl, but Tris caused selective release of catalase.These two types of microsomal catalase, i.e., that released by Tris (TS-released catalase) and that not released (TS-microsomal catalase), were labeled by injection of ^3H-leucine. A peak in the specific radioactivity appeared in the former at 50 min and in the latter at 30 min after the injection, and both thereafter lost their labels rapidly. Based on these results, a possible intracellular transition of catalase, TS-microsomal catalase &;DDotrahd TS-released catalase &;DDotrahd peroxisomal catalase, is discussed.
社団法人日本薬学会の論文
1986-04-25

Specific Release fo Rat Liver MIcrosomal Catalase

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