Isolation and Translation of Catalase mRNA of Bovine Liver

概要

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Messenger ribonucleic acid (mRNA) directing the biosynthesis of catalase [EC 1.11.1.6] was purified from bovine liver. The procedure consists of : (1) isolation of catalase-synthesizing polysomes by immunoprecipitation, (2) extraction of RNA from the polysomes with phenol, and (3) isolation of catalase mRNA by oligo (dT)-cellulose chromatography. Purified catalase mRNA has a molecular weight of 4-5×10^5 determined by electrophoresis on polyacrylamide-agarose gel. The mRNA stimulated catalase synthesis in a cell-free system derived from wheat germ ; i.e., the incorporation of ^<14>C-amino acids into catalase increased proportionally to the amount of mRNA added in the cell-free system and with incubation time. On SDS-polyacrylamide gel the radioactivity peak of the cell-free products precipitated by anti-catalase antibody coincided with the migration of carrier catalase. In translating system the mRNA preparation directed the incorporation of ^<14>C-amino acids into catalase at least 79% as efficiently as into total proteins.
社団法人日本薬学会の論文
1978-11-25