Studies on the Activity of α-Fetoprotein Gene

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概要

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• The expression of the gene of α-fetoprotein (AFP) was studied in AH-66 hepatoma cells and adult rat liver cells. With purified AFP mRNA as a template, [^3H]-labeled AFP complementary deoxyribonucleic acid (cDNA) (around 1000 nucleotides) was synthesized by the use of reverse transcriptase and was used for assaying the nucleotide sequence involved in AFP gene and in its transcripts by the hybridization technique. Upon treatment with DNase I, the AFP gene in AH-66 nuclei was digested preferentially, whereas that in rat liver nuclei was not. These results show that the AFP gene exists in a relaxed conformation in AH-66 but takes a more condensed structure in rat liver. Micrococcal nuclease selectively cleaved the AFP gene in AH-66,as did DNase I. It also preferentially recognized the AFP gene in rat liver, although to a lesser extent than that in AH-66. Chromosomal proteins extracted from AH-66 and ratliver chromatin were divided into four fractions with QAE-Sephadex A-25 (CP_<0,0.1,0.25 and 3> respectively). Liver chromatin was dissociated and reconstituted in the presence of each of AH-66 CP_<0-3> and, conversely, AH-66 chromatin was reconstituted in the presence of each of liver CP_<0-3>. Reconstituted chromatins were transcribed by E. coli RNA polymerase and the transcripts were annealed to AFP cDNA. AH-66 CP_<0.1> activated the AFP gene transcription strongly, while none of the liver CP_<0-3> inhibited the expression of the gene.

• 社団法人日本薬学会の論文
• 1981-11-25

著者

• 東 悳彦

  Department Of Biochemistry School Of Pharmaceutical Sciences Showa University

• 崔 南虎

  Department of Biochemistry, School of Pharmaceutical Sciences, Showa University

• 崔 南虎

  昭和大学 薬 生理化

関連論文

• Studies on the Activity of α-Fetoprotein Gene
• Isolation of α-Fetoprotein Messenger Ribonucleic Acid from AH-66 Ascites Hepatoma Cells
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• Effect of Ginseng Saponins on Cholesterol Metabolism. II. Effect of Ginsenosides on Cholesterol Synthesis by Liver Slice
• Stimulation of Serum Protein Synthesis in Ginsenoside Treated Rat
• Effect of Ginseng Saponins on Nuclear Ribonucleic Acid (RNA) Metabolism. I. RNA Synthesis in Rats treated with Ginsenosides
• Effect of Ginseng Saponins on Cholesterol Metabolism. I. The Level and the Synthesis of Serum and Liver Cholesterol in Rats treated with Ginsenosides
• Differential Effect of 3-Amino-1,2,4-triazole on Multiple Forms of Rat Liver Catalase
• Effect of Cycloheximide on Biosynthesis of Rat Liver Catalase
• Isolation and Translation of Catalase mRNA of Bovine Liver

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