Effect of Ginseng Saponins on Nuclear Ribonucleic Acid (RNA) Metabolism. II. RNA Polymerase Activities in Rats treated with Ginsenoside

概要

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The activities of ribonucleic acid (RNA) polymerases I, II and III in a sodium deoxycholate (DOC)-treated enzyme preparation have been differentially determined using three combinations of assay conditions (metal ion ; Mg^<2+> or Mn^<2+>, (NH_4)_2SO_4 and α-amanitin). Ginsenoside-Rb_1 enhanced, while -Rc repressed, the activities of RNA polymerases I and II, whereas both had no effect on that of RNA polymerase III. Rb_1-treated rats showed different profiles of stimulation of RNA polymerases I and II ; the maximum increase of RNA polymerase I activity was about +70% at 2 hr, and that of RNA polymerase II activity was +40% at 3 hr after the injection of Rb_1. Actinomycin D and cycloheximide both blocked the increase in RNA polymerase I activity on treatment with ginsenoside-Rb_1. On the other hand, increased activity of RNA polymerase II was blocked by actinomycin D but not by cycloheximide. These results suggest transcriptional regulation in the enhancement by ginsenoside-Rb_1 of RNA polymerase activities I and II, though the mechanisms may differ in detail.
社団法人日本薬学会の論文
1979-09-25